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Ensayos para estudiar el rol de vitronectina en la adhesión bacteriana y resistencia de suero
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
DOI:

10:55 min

October 16, 2018

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Chapters

  • 00:04Title
  • 00:49Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
  • 02:18ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
  • 04:11Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
  • 04:33Bacterial Adherence to the Vn-coated Glass Surfaces
  • 05:32Vn-dependent Adherence of Bacteria to Epithelial Cells
  • 07:29Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
  • 08:29Results: Interaction of Vitronectin and Bacteria
  • 10:10Conclusion

Summary

Automatic Translation

Este informe describe los protocolos para la caracterización de las interacciones entre proteínas de membrana externa bacteriana y la vitronectina de regulador del complemento humano. Los protocolos pueden utilizarse para estudiar las reacciones de enlace y función biológica de vitronectina en cualquier especie bacteriana.

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