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星爆无长突细胞的膜片钳在Deafferentated小鼠视网膜的平板安装准备
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Neuroscience
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JoVE Journal Neuroscience
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
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星爆无长突细胞的膜片钳在Deafferentated小鼠视网膜的平板安装准备

DOI:
10.3791/54608-v

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08:44 min

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October 13, 2016

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, , ,
1Department of Ophthalmology,Baylor College of Medicine, 2Department of Neuroscience,Baylor College of Medicine, 3Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:03Retina Dissection
  • 03:30Whole-cell Patch Clamp Recording from Flat-mount Retina
  • 06:34Results: Morphology and Membrane Potentials of ON-and Off-Starburst Amacrine Cell in the Nob Mouse Retina
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

此协议演示了如何从一台安装在准备视网膜神经元进行全细胞膜片钳记录。

Tags

Patch ClampStarburst Amacrine CellsFlat-mountDeafferentated Mouse RetinaRetinal NeurobiologyRetinal CircuitsWhole-cell RecordingNitrocellulose MembraneExternal SolutionCarbogenatedUpright Microscope

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