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Journal
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Immunology and Infection
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制备及胸腺器官切片文化中的应用
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Preparation and Applications of Organotypic Thymic Slice Cultures
Please note that all translations are automatically generated.
Click here for the English version.
制备及胸腺器官切片文化中的应用
DOI:
10.3791/54355-v
•
10:10 min
•
August 06, 2016
•
Aditi Sood*
1,2
,
Mengqi Dong*
1,2
,
Heather J. Melichar
3
1
Centre de Recherche
,
Hôpital Maisonneuve-Rosemont
,
2
Department of Microbiology, Infectiology and Immunology
,
Université de Montréal
,
3
Department of Medicine
,
Université de Montréal
Chapters
00:05
Title
00:45
Harvest of Mouse Thymus
01:52
Agarose Embedding and Vibratome Sectioning of Thymic Lobes
05:30
Preparation of Thymocytes for Overlaying
06:59
Overlaying Thymocytes onto the Thymic Slices
07:48
Results: Positive and Negative Selection of Thymocytes
09:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述的是,在用流式细胞仪组合,可以被用来建模显影T细胞的阳性和阴性选择胸腺切片的制备方法。胸腺片也可以适用于胸腺细胞迁移,本地化的
原位
分析,并通过免疫荧光和双光子显微镜信令。
Tags
Organotypic Thymic Slice Cultures
T Cell Development
Positive And Negative Selection
Thymic Cortex And Medulla
Thymocyte Subsets
Thymic Microenvironment
Mouse Thymus Dissection
Agarose Embedding
RPMI-1640 Media
Cell Culture Inserts
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