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福尔马林固定石蜡包埋的组织核心的制备RNA和DNA提取
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
Please note that all translations are automatically generated.
Click here for the English version.
福尔马林固定石蜡包埋的组织核心的制备RNA和DNA提取
DOI:
10.3791/54299-v
•
08:30 min
•
August 21, 2016
•
Palak G. Patel
2
,
Shamini Selvarajah
2
,
Suzanne Boursalie
2
,
Nathan E. How
2
,
Joshua Ejdelman
,
Karl-Philippe Guerard
,
John M. Bartlett
,
Jacques Lapointe
,
Paul C. Park
,
John B. A. Okello
2
,
David M. Berman
2
1
Department of Pathology & Molecular Medicine
,
Queen’s University
,
2
Division of Cancer Biology & Genetics, Queen’s Cancer Research Institute
,
Queen’s University
,
3
Department of Surgery, Division of Urology
,
McGill University
,
4
Transformative Pathology Program
,
Ontario Institute for Cancer Research (OICR)
Chapters
00:05
Title
00:57
Tissue Coring
02:40
Deparaffinization of the FFPE Tissue Cores
03:45
Homogenization of the Deparaffinized Cores
05:07
Digestion with Proteinase K and Nucleotide Separation
06:12
Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
07:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个修改后的提取方法提高了从组织病理学组织块的兴趣更精确的目标区域的RNA和DNA的产量。
Tags
RNA Extraction
DNA Extraction
Prostate Cancer
Paraffin Film
Punch Biopsy
Xylene
Centrifugation
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