Caracterização de derivados de monócitos células dendríticas humanas por imagem Citometria de Fluxo: Uma Comparação entre dois protocolos de isolamento de monócitos
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.