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1Molecular Imaging and Neurovascular Research Laboratory,Dongguk University College of Medicine, 2Biomedical Research Center,Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering,Seoul National University, 4Departments of Radiology and Cancer Systems Imaging,University of Texas M.D. Anderson Cancer Center
Chapters
- 00:05Title
- 01:20Exogenously Formed Clot Preparation and Fluorescent Marker Labeling
- 02:55Thromboembolic Stroke Model Preparation
- 05:42In Vivo MicroCT Cerebral Thrombus Imaging
- 06:36Ex Vivo Near Infrared Fluorescent (NIRF) Thrombus Imaging and MicroCT Thrombus Area Imaging Quantification
- 09:48Results: Representative Direct Cerebral Thrombus Imaging before and after Thrombolysis Activation
- 11:28Conclusion
Este protocolo descreve a aplicação de tomografia combinada fluorescente no infravermelho próximo (NIRF) e imagiologia de micro-computadorizada (microCT) para visualizar tromboêmbolos cerebral. Esta técnica permite a quantificação da carga de trombo e evolução. A técnica de imagem NIRF visualiza fluorescente etiquetado trombo no cérebro extirpado, enquanto que a técnica microCT visualiza trombo no interior de animais vivos usando ouro-nanopartículas.
