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详细的定性C1498小鼠细胞系及其关联白血病小鼠模型协议
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
详细的定性C1498小鼠细胞系及其关联白血病小鼠模型协议
DOI:
10.3791/54270-v
•
08:00 min
•
October 14, 2016
•
Alexia Mopin
,
Virginie Driss
,
Carine Brinster
2
1
Institut pour la Recherche sur le Cancer de Lille (IRCL)
,
INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC)
,
2
Université de Lille
Chapters
00:05
Title
00:53
Intravenous C1498 Injection and Retro-orbital Blood Collection
02:15
Blood Mononuclear Cells Isolation
03:11
Bone Marrow Cells Isolation
04:41
Bone Marrow Cells Preparation on Slides and May-Grünwald Giemsa staining
06:06
Representative Results: C1498 Acute Myeloid Leukemia Characterization
07:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该原稿提供了可用于表征
在体外
和诱导小鼠的注射后急性白血病C1498细胞培养技术的过程。表型和功能分析使用流式细胞仪,免疫荧光显微镜,细胞化学和五月 - 格伦沃尔德姬姆萨染色进行。
Tags
C1498 Cells
Murine Myelomonocytic Leukemia
Acute Leukemia
Animal Model
Congenic C57 Black 6 Mouse
Tail Vein Injection
Orbital Sinus Blood Draw
Mononuclear Cell Isolation
Flow Cytometry
Bone Marrow Isolation
Leukemia Development
Immune Deficiency
Chemo And Immunotherapeutic Agents
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