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从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析
JoVE Journal
Medicine
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JoVE Journal
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Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
Please note that all translations are automatically generated.
Click here for the English version.
从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析
DOI:
10.3791/54259-v
•
07:10 min
•
July 29, 2016
•
David J. Agorku
,
Stefan Tomiuk
,
Kerstin Klingner
,
Stefan Wild
,
Silvia Rüberg
,
Lisa Zatrieb
,
Andreas Bosio
,
Julia Schueler
,
Olaf Hardt
1
R&D Reagents
,
Miltenyi Biotec GmbH
,
2
In vivo Tumorbiology
,
Oncotest GmbH
Chapters
00:05
Title
00:40
Tumor Dissociation
02:06
Magnetic Cell Separation Mouse Cell Depletion
04:01
Representative Results: Downstream Mouse Cell-free Human Tumor Cell Analyses
05:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
人类肿瘤异种移植是血管,并在
体内
生长阶段由鼠源细胞浸润。为了规避下游分析过程中造成这些污染的细胞偏差,我们开发了允许通过磁性细胞分选所有小鼠细胞的全面枯竭的方法。
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