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Journal
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Immunology and Infection
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高通量设置的建立筛选小分子能调节C-二GMP信令<em>绿脓杆菌</em
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in
Pseudomonas aeruginosa
Please note that all translations are automatically generated.
Click here for the English version.
高通量设置的建立筛选小分子能调节C-二GMP信令<em>绿脓杆菌</em
DOI:
10.3791/54115-v
•
11:31 min
•
June 30, 2016
•
Kushal N. Rugjee
,
Shi-qi An
,
Robert P. Ryan
1
Division of Molecular Microbiology, College of Life Sciences
,
University of Dundee
Chapters
00:05
Title
00:59
Generation of GFP Tagged c-di-GMP Reporter
P. aeruginosa
Strain
04:21
Preparation of the Starter Culture for Inoculation
05:35
Inoculation and Incubation of the 384-well Plates Containing Small Molecules
06:33
Measurement and Analysis of Growth and Intracellular c-di-GMP Level
08:25
Results: Hits Obtained Following the High-throughput Screen against
P. aeruginosa
10:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了已经成功地建立筛选小分子的大文库其操纵在
绿脓杆菌
环状二-GMP的细胞水平的潜在能力,提供了抗菌药物发现和化合物测试一个新的强有力的工具的高通量测定法。
Tags
High-throughput
Screening
Small Molecules
Modulate
C-di-GMP
Pseudomonas Aeruginosa
Second Messenger
Bio-reporter
Cyclic-di-GMP
Novel Therapies
Immune System
Antibiotics
Electroporation
CdrA Promoter
Green Fluorescent Protein
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