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设计和适体 - 金纳米粒子基于比色法的开发在这领域的应用
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JoVE Journal Biology
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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设计和适体 - 金纳米粒子基于比色法的开发在这领域的应用

DOI:
10.3791/54063-v

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08:23 min

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June 23, 2016

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, , ,
1Department of Science and Mathematics,Alvernia University, 2711th Human Performance Wing, Air Force Research Laboratory,Wright-Patterson Air Force Base, 3UES, Inc.

Chapters

  • 00:05Title
  • 00:47Synthesis via Citrate Reduction of Gold Nanoparticles (AuNP) and Characterization
  • 02:35DNA-aptamer, Buffer, Solution, and Assay Preparation
  • 03:35Salt Titration and Assay Setup
  • 05:21Freezing Aptamer–AuNP Assay for Long-term Storage
  • 06:38Results: Assay Response with Changing DNA Coverage Density and Performance of the Assay for Long-term Storage
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

进行了检查适体，纳米金比色法检测小分子在得到了实地应用的设计与开发。此外，一个智能设备色度应用（应用程序）的验证，并建立了在该领域使用的测定法的长期贮存。

Tags

AptamerGold NanoparticleColorimetric AssayIn-field ApplicationReproducibilityShelf-lifeFalse PositiveGold ChlorideSodium CitrateDEPCAutoclaveCocaine-binding AptamerPhosphoramidite ChemistryOligonucleotide

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