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Journal
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Immunology and Infection
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该<em>离体</em>文化与模式识别小鼠肠道组织体受体刺激
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
The
Ex Vivo
Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
Please note that all translations are automatically generated.
Click here for the English version.
该<em>离体</em>文化与模式识别小鼠肠道组织体受体刺激
DOI:
10.3791/54033-v
•
09:09 min
•
May 18, 2016
•
Daniel E. Rothschild
,
Tara Srinivasan
,
Linette A. Aponte-Santiago
,
Xiling Shen
3,4
,
Irving C. Allen
1
Department of Biomedical Sciences and Pathobiology
,
Virginia Maryland College of Veterinary Medicine, Virginia Tech
,
2
Department of Biomedical Engineering
,
Cornell University
,
3
School of Electrical and Computer Engineering
,
Cornell University
,
4
Department of Biomedical Engineering
,
Duke University
Chapters
00:05
Title
00:46
Mus musculus
Small Intestinal Crypt Harvest
03:57
Organoid Plating for Pathogen Associated Molecular Patters (PAMP) and
L. monocytogenes
Challenge
06:58
Results: Representative Mouse Intestinal Organoid Culture and Stimulation
08:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里,收获,维护和治疗与病原体相关的分子模式(PAMP),并
李斯特菌
小鼠小肠组织体的协议被描述,以及强调基因表达和蛋白质正确规范化技术。
Tags
Mouse Intestinal Organoids
Ex Vivo Culture
Pattern Recognition Receptor Stimulation
Innate Immunity
Mucosal Immunology
Host-pathogen Interactions
Intestinal Epithelial Cells
ELISA
Intestinal Crypts
Normalization
Cell Number
PBS
EDTA
DMEM/F12
Cell Fractionation
Crypt Visualization
Article
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