Ontwikkeling en karakterisering van<em> In Vitro</em> Microvaatje Netwerk- en kwantitatieve metingen van endotheliale [Ca<sup> 2+</sup>]<sub> i</sub> En productie van stikstofoxide
Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.