JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
巨蟹座的建立源于人类传统的骨肉瘤细胞培养
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Please note that all translations are automatically generated.
Click here for the English version.
巨蟹座的建立源于人类传统的骨肉瘤细胞培养
DOI:
10.3791/53884-v
•
09:25 min
•
October 14, 2016
•
Gaia Palmini
,
Roberto Zonefrati
,
Carmelo Mavilia
,
Alessandra Aldinucci
,
Ettore Luzi
,
Francesca Marini
,
Alessandro Franchi
,
Rodolfo Capanna
,
Annalisa Tanini
,
Maria Luisa Brandi
1
Department of Surgery and Translational Medicine (DCMT)
,
University of Florence
,
2
Neurofarba Department
,
University of Florence
,
3
Department of Traumatology and General Orthopedics
,
Azienda Ospedaliera Universitaria Careggi
Chapters
00:05
Title
01:20
Establishment of an Osteosarcoma Cell Line and the Sarcosphere Assay
03:12
Isolation of Sarcospheres
05:48
In Vitro Analysis to Characterize the Osteosarcoma Cancer Stem Cells
06:24
Results: Establishing a Verified Culture of Cancer Stem Cells
07:55
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
癌症干细胞的骨肉瘤存在(的CSCs)最近被链接到他们的发病机理。在这篇文章中,我们目前使用的CSC不依条件下生长的能力从传统的骨肉瘤(OS)的人获得活检原代细胞培养肿瘤干细胞的分离。
Tags
Cancer Stem Cells
Osteosarcoma
Sarcosphere
In Vitro
Cell Culture
Cell Isolation
Cell Suspension
Growth Factors
EGF
BFGF
Ultra-low Attachment Plates
Hemocytometer
Phase Contrast Microscopy
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
骨肉瘤前小鼠模型对肿瘤与间充质干细胞细胞外囊泡介导通信的定义
应用 Fraumeni 综合征诱导多潜能干细胞建模骨肉瘤
多端根结核细胞迁移的实时监测
建立胃癌患者衍生异种移植模型和原细胞系
从妇科和乳腺癌肿瘤获得癌症干细胞球
从3维球体培养中分离出类似干细胞的细胞
使用腺相关病毒-Cas9系统在Vitro建立基因工程的毛因头和颈部癌细胞系
在结肠直肠癌细胞的三维球体培养物中使用无细胞益生菌超常剂评估细胞死亡
从细胞和组织样本中分离和功能评估人类乳腺癌干细胞
来自分离人产品的γ-Delta(γδ)T细胞的扩增和富集
Read Article