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一个简单的生物测定血管内皮生长因子的评价
JoVE Journal
Biology
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JoVE Journal Biology
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
DOI:

09:04 min

March 15, 2016

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Chapters

  • 00:05Title
  • 00:46Preparation of WEHI-3D-Conditioned Medium Containing IL-3
  • 02:03Culture and Evaluation of VEGFR-2-EpoR-Ba/F3 and Control Ba/F3 Cell Lines
  • 04:09Semi-quantitative Assay
  • 06:17Quantitative Bioassay
  • 06:59Results: Inhibition of the Effects of VEGF-A165 by Bevacizumab
  • 08:12Conclusion

Summary

Automatic Translation

我们描述了检测,定量和监测血管内皮生长因子家族的配体的成员的活性的简单的基于细胞的生物测定法。该测定法使用在因子相关性细胞系中表达的嵌合受体的配体,以提供受体结合和交联的半定量或定量的评估。

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