JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
一个简单的生物测定血管内皮生长因子的评价
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Please note that all translations are automatically generated.
Click here for the English version.
一个简单的生物测定血管内皮生长因子的评价
DOI:
10.3791/53867-v
•
09:04 min
•
March 15, 2016
•
Steven A. Stacker
,
Michael M. Halford
,
Sally Roufail
,
Carol Caesar
,
Marc G. Achen
1
Tumour Angiogenesis Program
,
Peter MacCallum Cancer Centre
Chapters
00:05
Title
00:46
Preparation of WEHI-3D-Conditioned Medium Containing IL-3
02:03
Culture and Evaluation of VEGFR-2-EpoR-Ba/F3 and Control Ba/F3 Cell Lines
04:09
Semi-quantitative Assay
06:17
Quantitative Bioassay
06:59
Results: Inhibition of the Effects of VEGF-A
165
by Bevacizumab
08:12
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们描述了检测,定量和监测血管内皮生长因子家族的配体的成员的活性的简单的基于细胞的生物测定法。该测定法使用在因子相关性细胞系中表达的嵌合受体的配体,以提供受体结合和交联的半定量或定量的评估。
Tags
Vascular Endothelial Growth Factor
VEGF
Bioassay
Cell-based Assay
VEGFR
IL3
WEHI-3D
BAF3
VegFR2-EPoR-BAF3
Cell Culture
Cell Washing
Cell Preparation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
一个简单的协议提取血细胞从野生毛毛虫
简单和高效的睾丸细胞悬液的制备技术
鼠阀门内皮细胞的分离
隔离和鼠标主动脉内皮细胞原代培养
鼠冠状动脉血管平滑肌细胞的分离
核酸的分离过滤:一种简单快速的DNA提取方法
转化生长因子β信号诱导内皮-间质转移的分子分析
使用 En 面免疫荧光染色直接观察血管内皮细胞
在肾脏皮质提取物中演示血管内皮生长因子与叶黄素激素之间的线性关系
分离人原代瓣膜细胞进行体外疾病建模
Read Article