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体外
肺转移及微环境的实时成像
JoVE Journal
Medicine
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JoVE Journal
Medicine
Ex vivo
Live Imaging of Lung Metastasis and Their Microenvironment
Please note that all translations are automatically generated.
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体外
肺转移及微环境的实时成像
DOI:
10.3791/53741-v
•
11:19 min
•
February 03, 2016
•
Renske J.E. van den Bijgaart*
1
,
Niwen Kong*
1
,
Carrie Maynard
2
,
Vicki Plaks
3
1
Department of Anatomy
,
University of California
,
2
Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht
,
3
Department of Cell and Developmental Biology
,
Sackler Faculty of Medicine
Chapters
00:05
Title
01:03
Lung Preparation for Ex vivo Live Imaging
05:16
Image Acquisition and Analysis
08:36
Results: Ex vivo Imaging of Lung Metastasis and Their Microenvironment
10:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述
用于
离体肺转移中的肿瘤细胞-基质相互作用的实时成像的相对简单的方法,利用荧光报告小鼠。使用纺丝盘共焦显微镜,该技术使活细胞的可视化的至少4小时,并可以适用于研究其它炎性肺部疾病。
Tags
Ex Vivo Live Imaging
Lung Metastasis
Tumor Cell Stroma Interactions
Metastasis Establishment
Cell Types
Microenvironment
Targeted Therapeutics
Lung Imaging
Cell Death
Mouse
Trachea
Agarose
Article
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