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流式细胞仪用来评估染色质的T细胞国家
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
The Use of Flow Cytometry to Assess the State of Chromatin in T Cells
DOI:

11:01 min

December 17, 2015

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Chapters

  • 00:05Title
  • 00:40Single Cell Suspension of Lymphocytes from Mouse Spleens
  • 05:39Viability and Surface Staining
  • 07:55Intracellular Staining for Histone H3
  • 09:42Results: Assessing the State of Chromatin in T Cells
  • 10:30Conclusion

Summary

Automatic Translation

流式细胞仪可用于T细胞内的染色质评估的状态。该协议允许科学家在T细胞活化的增加的荧光组蛋白H3的抗体的平均荧光强度(MFI)证明解释染色质解聚的证据

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