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Journal
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Immunology and Infection
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流式细胞仪用来评估染色质的T细胞国家
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
The Use of Flow Cytometry to Assess the State of Chromatin in T Cells
Please note that all translations are automatically generated.
Click here for the English version.
流式细胞仪用来评估染色质的T细胞国家
DOI:
10.3791/53533-v
•
11:01 min
•
December 17, 2015
•
Kellie N. Bingham*
1
,
Megan D. Lee*
1
,
Jason S. Rawlings
1
Department of Biology
,
Furman University
Chapters
00:05
Title
00:40
Single Cell Suspension of Lymphocytes from Mouse Spleens
05:39
Viability and Surface Staining
07:55
Intracellular Staining for Histone H3
09:42
Results: Assessing the State of Chromatin in T Cells
10:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
流式细胞仪可用于T细胞内的染色质评估的状态。该协议允许科学家在T细胞活化的增加的荧光组蛋白H3的抗体的平均荧光强度(MFI)证明解释染色质解聚的证据
Tags
Flow Cytometry
Chromatin
T Cells
Activation
Histone H3
Cell Viability
Antigen Presentation
Clonal Proliferation
Chromatin Decondensation
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