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Immunology and Infection
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益生菌酵母的转型及其胃肠道粘膜免疫组织恢复继小鼠口服灌胃
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
Please note that all translations are automatically generated.
Click here for the English version.
益生菌酵母的转型及其胃肠道粘膜免疫组织恢复继小鼠口服灌胃
DOI:
10.3791/53453-v
•
12:12 min
•
February 08, 2016
•
Lauren E. Hudson
,
Taryn P. Stewart
,
Milo B. Fasken
,
Anita H. Corbett
,
Tracey J. Lamb
1
Department of Pediatrics
,
Emory University School of Medicine
,
2
Department of Biochemistry
,
Emory University School of Medicine
Chapters
00:05
Title
01:00
Generate Survival Curves to Determine Needed Doses of UV Irradiation
03:01
Screening for Mutagenized Auxotrophic Yeast Strains and Yeast Transformation
05:42
Oral Gavage of Mice with Transformed Yeast
07:50
Harvest of Murine Peyer’s Patches and Isolation of Viable Yeast Colonies
10:03
Representative Results
11:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是,可以用于开发,变换,管理,和测试益生菌酵母
布拉酵母菌
中异源蛋白质表达技术的统一描述。
Tags
Probiotic Yeast
Oral Gavage
UV Irradiation
5-FOA
Ura3 Mutant
Oxytrophic Strains
Genetic Manipulation
Oral Vaccination
Gastrointestinal Therapeutics
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