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Neuroscience
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人 Intraepidermal 神经纤维内线粒体三维成像及分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
Please note that all translations are automatically generated.
Click here for the English version.
人 Intraepidermal 神经纤维内线粒体三维成像及分析
DOI:
10.3791/53369-v
•
10:31 min
•
September 29, 2017
•
Hussein S. Hamid
,
John M. Hayes
,
Eva L. Feldman
,
Stephen I. Lentz
1
University of Michigan Medical School
,
2
Department of Neurology
,
University of Michigan
,
3
Department of Internal Medicine
,
University of Michigan
Chapters
00:05
Title
01:02
Fluorescence Immunohistochemistry: Day 1
02:27
Fluorescence Immunohistochemistry: Day 2
03:21
Fluorescence Immunohistochemistry: Day 3
04:22
Confocal Imaging
06:00
Image Processing
07:03
Isolation of Nerve-specific Mitochondrial Signal
08:41
Results: Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
09:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议使用三维 (3D) 成像和分析技术来可视化和量化神经特异性线粒体。这些技术适用于在其他情况下, 一个荧光信号被用来隔离一个子集的数据, 从另一个荧光信号。
Tags
3D Imaging
Mitochondria
Intraepidermal Nerve Fibers
Fluorescence Immunohistochemistry
PGP9.5
PDH
Secondary Antibodies
Signal Enhancement
Epidermal Nerve Fibers
Neurological Complications
Article
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