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对小鼠乳腺冰冻切片间接免疫荧光
JoVE Journal
Biology
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JoVE Journal Biology
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
DOI:

11:13 min

December 01, 2015

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Chapters

  • 00:05Title
  • 00:42Mouse Mammary Gland Dissection
  • 01:56Mammary Tissue Fixation and Embedding
  • 03:23Frozen Tissue Sectioning
  • 05:20Indirect Immunofluorescence
  • 08:47Results: Mapping Proteins Involved with Lactation
  • 09:53Conclusion

Summary

Automatic Translation

在这篇文章中所描述的间接免疫荧光协议允许的检测和蛋白在小鼠乳腺定位。一个完整的方法是考虑到制备乳腺样品,利用荧光显微镜进行免疫组织化学,将图像的组织切片,并重建图像。

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