JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
对小鼠乳腺冰冻切片间接免疫荧光
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Please note that all translations are automatically generated.
Click here for the English version.
对小鼠乳腺冰冻切片间接免疫荧光
DOI:
10.3791/53179-v
•
11:13 min
•
December 01, 2015
•
Edith Honvo-Houéto
,
Sandrine Truchet
1
Jouy-en-Josas Centre
,
INRA
Chapters
00:05
Title
00:42
Mouse Mammary Gland Dissection
01:56
Mammary Tissue Fixation and Embedding
03:23
Frozen Tissue Sectioning
05:20
Indirect Immunofluorescence
08:47
Results: Mapping Proteins Involved with Lactation
09:53
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这篇文章中所描述的间接免疫荧光协议允许的检测和蛋白在小鼠乳腺定位。一个完整的方法是考虑到制备乳腺样品,利用荧光显微镜进行免疫组织化学,将图像的组织切片,并重建图像。
Tags
Indirect Immunofluorescence
Mouse Mammary Gland
Protein Detection
Frozen Sectioning
Antigen Retrieval
Image Acquisition
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用荧光抗体对小鼠表皮石蜡切片的免疫组织化学
小鼠乳腺上皮细胞形成过程中Lactogenic分化的微球体
乳腺上皮移植手术
变化在大鼠乳腺形态和患乳腺癌的风险
石蜡包埋及冷冻的部分
果蝇
成人肌肉
鼠标涎腺干细胞的分离
乳腺的发育和功能在小鼠模型的评价
成年小鼠心肌细胞的单细胞转录谱
导管内注射局部给药的小鼠乳腺
男性附属性腺产品对腹足软体动物下蛋的影响
Read Article