JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
使用拉姆齐试验来测量液体分泌和离子流率在<em>果蝇</em>马氏管
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the
Drosophila melanogaster
Malpighian Tubule
Please note that all translations are automatically generated.
Click here for the English version.
使用拉姆齐试验来测量液体分泌和离子流率在<em>果蝇</em>马氏管
DOI:
10.3791/53144-v
•
13:30 min
•
November 25, 2015
•
Jeffrey N. Schellinger
,
Aylin R. Rodan
1
Department of Internal Medicine
,
University of Texas Southwestern Medical Center
Chapters
00:05
Title
00:53
Preparing Tubule Transfer Rods
01:33
Preparing the Ion-Specific Electrodes
03:38
Preparing the Reference Electrodes and Calibrating the Electrodes
07:16
Tubule Dissection
09:41
Making Measurements
11:06
Results: Measurement K
+
and Na
+
Ion Fluxes to Investigate the Role of the Sodium-potassium-2-chloride Cotransporter (NKCC)
12:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这个协议描述了使用的拉姆齐测定来测量流体分泌率从
果蝇
孤立马氏(肾)细管。此外,使用离子特异性电极来测量分泌的流体钠和钾的浓度,允许跨上皮离子通量的计算,进行说明。
Tags
Ramsay Assay
Fluid Secretion
Ion Flux
Drosophila Melanogaster
Malpighian Tubule
Epithelial Ion Transport
Transepithelial Fluid
Ion-specific Electrodes
Sodium
Potassium
Genetics
Molecular Mechanisms
Homeostasis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在果蝇幼虫中枢神经系统的剖析
研究有丝分裂的显微注射技术
果蝇
合胞体胚胎
克隆形成含量:贴壁细胞
漂白实验(FRAP和FLIP)来衡量生活胚胎干细胞的染色质的蛋白质动力学
注射胰岛素和血淋巴的提取来衡量成人胰岛素敏感性
果蝇</em
寿命测量<em>果蝇</em
凋亡细胞清除过程中实时成像<em>果蝇</em>胚胎发育
通过影像学的蛹壳<em>果蝇</em
定量免疫检测,以衡量在中心体的变异蛋白水平
用荧光基因编码的 ph 指示剂对
果蝇
氏小管上皮细胞内 ph 值的光学定量
Read Article