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Journal
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Immunology and Infection
/
方法来提高高分辨率的敏感性疟疾熔单核苷酸多态性基因分型
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Please note that all translations are automatically generated.
Click here for the English version.
方法来提高高分辨率的敏感性疟疾熔单核苷酸多态性基因分型
DOI:
10.3791/52839-v
•
10:27 min
•
November 10, 2015
•
Rachel Daniels
2
,
Elizabeth J. Hamilton
,
Katelyn Durfee
,
Daouda Ndiaye
,
Dyann F. Wirth
5
,
Daniel L. Hartl
,
Sarah K. Volkman
4
1
Department of Organismic and Evolutionary Biology
,
Harvard University
,
2
Department of Immunology and Infectious Diseases
,
Harvard T.H. Chan School of Public Health
,
3
Faculty of Medicine and Pharmacy
,
Cheikh Anta Diop University
,
4
School of Nursing and Health Sciences
,
Simmons College
,
5
Institute of Infectious Diseases
,
Broad Institute
Chapters
00:05
Title
01:58
Template Preparation
03:46
PCR Amplification
05:37
HRM Analysis
08:26
Results: Blocked-probe-based and MAAB Increases HRM Genotyping Sensitivity
09:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
虽然高分辨率熔解分析提供了一个异质群体的单核苷酸多态性区分的能力,突变等位基因扩增偏置可提高其检测存在于样品中相对较低的百分比等位基因的能力。本协议描述的改进,提高了高分辨率熔解分析的灵敏度。
Tags
Malaria
Plasmodium
Single-nucleotide Polymorphisms (SNPs)
Drug Resistance
Population Surveillance
High-resolution Melting (HRM) Analysis
Polygenomic Infections
PCR Amplification
Minor Allele
Genotyping Sensitivity
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