JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Please note that all translations are automatically generated.
Click here for the English version.
长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
DOI:
10.3791/52833-v
•
15:57 min
•
July 11, 2015
•
Mayara F. Maggioli
2
,
Mitchell V. Palmer
,
H. Martin Vordermeier
,
Adam O. Whelan
,
James M. Fosse
,
Brian J. Nonnecke
,
W. Ray Waters
1
Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service
,
United States Department of Agriculture
,
2
Department of Veterinary Pathology, College of Veterinary Medicine
,
Iowa State University
,
3
UK Veterinary Laboratories Agency
,
4
Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service
,
United States Department of Agriculture
Chapters
00:00
Title
01:40
Methods
02:05
Long-term Culture
03:38
Elispot Assay Day 1
04:30
Elispot Assay Day 2
05:15
Adherent Cell Isolation
05:37
Flow Cytometry Plate Preparation
06:15
Harvest the 13 Day Long-term Cells
09:05
Elispot Assay Day 3
10:04
Alkaline Phosphatase Preparation
11:08
Substrate Solution Preparation
11:55
Reading the Plate
12:12
Performing Flow Cytometry
12:25
Results
14:37
Conclusions
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
长期培养的干扰素γ酶联免疫斑点法被用作中央记忆应答的量度并且与保护性抗分枝杆菌疫苗的反应。用该测定中,外周血单核细胞刺激与分枝杆菌抗原和IL-2 14天,使分化和扩展中央记忆T细胞。
Tags
Interferon-γ
ELISPOT Assay
Effector T Cells
Memory T Cells
Central Memory T Cells
Effector Memory T Cells
Cattle
Tuberculosis
Vaccination
Antigen Stimulation
Long-term Culture
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
酶联免疫斑点法(ELISPOT):TH - 1细胞对微生物抗原的免疫应答的定量
使用γ-干扰素酶酶联免疫检测的特点人类乳头状瘤病毒的新型T-细胞表位
在体外试验
,以评估免疫调节途径对HIV特异性CD4 + T细胞效应功能的影响
荧光目标阵列的T细胞反应评价中的应用<em>在体内</em
通过酶联凝集素测定来测量神经氨酸酶流感抑制抗体滴度
IL-8瞬时转化的小鼠模型<em>体内</em>长期监测炎症反应
慢性自身免疫性干眼鼠模型增加效应记忆T细胞在眼球组织
巨噬细胞中含有吞噬的
鼠伤寒沙门氏菌
的分离
双光子激光显微镜在细胞膜修复中的应用
一种研究人体 T 细胞召回反应
的
吸水泡协议
Read Article