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方法培养人股骨组织外植体，究其利基转移性乳腺癌细胞定居
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Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche
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方法培养人股骨组织外植体，究其利基转移性乳腺癌细胞定居

DOI:
10.3791/52656-v

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11:41 min

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March 15, 2015

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, , , , ,
1Department of Pediatrics,Stanford University School of Medicine, 2Department of Orthopaedic Surgery,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 02:00Isolating Tissue Fragments from Femoral Heads
  • 02:40Co-culturing to Study Cell Proliferation
  • 04:19Co-culturing to Study Colonization and Cell Number
  • 06:45Measuring Cell Migration in the Co-culture: Towards Bone Tissue Supernatant
  • 09:30Results: Breast Cancer Cell Proliferation, Colonization, and Migration in Co-culture
  • 11:10Conclusion

Summary

Automatic Translation

Automatic Translation

协议描述为研究乳腺癌细胞的迁移，增殖和定植于人体骨组织外植体模型系统。

Tags

Bone MetastasisBreast CancerIn Vitro ModelMicroenvironmentMetastatic NicheCancer Cell ColonizationBioluminescence ImagingFluorescence MicroscopyFlow Cytometry

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