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人掌腱膜挛缩的<em>离体</em>文化为肌成纤维细胞的研究和细胞外基质相互作用
JoVE Journal
Medicine
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JoVE Journal Medicine
Human Dupuytren’s Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
DOI:

08:59 min

April 18, 2015

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Chapters

  • 00:05Title
  • 02:06Tissue Preparation and Ex Vivo Tissue Culture Setup
  • 05:27Combined Second Harmonic Generation (Collagen) and Two-photon Excited Fluorescence (Elastin) Imaging on Ex Vivo Tissue during Culture
  • 06:39Results: Effects on 3D Extracellular Matrix Composition upon Tissue Manipulation by Compound Treatments and Viral-mediated Gene Modulation
  • 08:17Conclusion

Summary

Automatic Translation

迪皮特朗病(DD)是手的手掌的纤维增生性疾病。在这里,我们提出了一个协议,从DD文化切除标本的三维(3D)培养体系。这种短期培养系统允许保存的三维结构和纤维化组织的分子性质。

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