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从细胞亚群特异性RNA提取使用激光捕获显微切割加上快速的免疫标记
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Biology
RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
Please note that all translations are automatically generated.
Click here for the English version.
从细胞亚群特异性RNA提取使用激光捕获显微切割加上快速的免疫标记
DOI:
10.3791/52510-v
•
07:01 min
•
April 11, 2015
•
Audrey Chabrat*
1,2
,
Hélène Doucet-Beaupré*
1,2
,
Martin Lévesque
2
1
Department of Psychiatry and Neurosciences, Faculty of Medicine
,
Université Laval
,
2
Centre de recherche de l’Institut universitaire en santé mentale de Québec
Chapters
00:05
Title
01:25
Sample Sectioning
02:28
Staining
04:06
Laser Capture Microdissection (LCM)
05:14
Results: Representatve LCM-isolated RNA Analysis
06:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞在特定组织的一个子集的基因表达分析是一个重大的挑战。本文介绍了如何通过将快速免疫标记方法与激光捕获显微切割分离,从一个特定的细胞群高质量的总RNA。
Tags
Laser Capture Microdissection
LCM
RNA Isolation
Immunolabeling
Cell-specific Subpopulations
Dopaminergic Cells
RNA Integrity
RNA-sequencing
Microarray
QRT-PCR
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