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Untersuchung Mast Cell Sekretgranula; von Biosynthese zu Exocytosis
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1Department of Cell and Developmental Biology,Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences,Graduate School of Life Sciences, Tohoku University
Chapters
- 00:05Title
- 02:37Transfection of Rat Basophilic Leukemia (RBL) Cells
- 06:06Measuring NPY mRFP Exocytosis
- 07:48Time-lapse Microscopy of Exocytosis
- 10:51Image Analyses
- 13:00Results: Quantifying Mast Cells Secretory Granule (SG) Size and their Exocytosis
- 14:44Conclusion
Das Ziel des vorliegenden Protokolls war es, eine Methode, die funktionelle Genomanalysen von Mastzellen Sekretion ermöglichen entwickeln wird. Das Protokoll basiert auf einer quantitativen Beurteilung der Freisetzung eines fluoreszierenden Reporter-Gen mit dem Gen von Interesse und den Echtzeit cotrasfected Analysen der sekretorischen Granula Morphologie.
