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使用李斯特菌单细胞基因评估受感染小鼠培养和心脏殖民化中心脏细胞的细菌入侵
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
DOI:

09:18 min

May 27, 2015

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Chapters

  • 00:05Title
  • 01:54Invasion Assay
  • 04:09Inoculating L. monocytogenes into Mice via Tail Vein Injection and Assessing Bacterial Burden in Liver, Spleen, and Heart
  • 07:39Results: L. monocytogenes Infection in Cultured Organ Cells
  • 08:47Conclusion

Summary

Automatic Translation

李斯特菌单细胞基因 导致孕妇胎儿感染和易感人群脑膜炎。 细菌的亚种群可以殖民心脏组织,导致患者和实验室动物心肌炎。 在这里,我们提出了一个协议,描述了如何评估 L.单细胞基因 心脏细胞 入侵体外 和心脏殖民化感染动物。

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