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케모카인 시그널링에 의해 유도 된 세포 및 분자 양극화의 신속하고 강력한 분석
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
DOI:

10:03 min

December 12, 2014

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Chapters

  • 00:05Title
  • 01:32Chemokine Stimulation
  • 02:41Cell Staining
  • 04:14Image Acquisition and Analysis
  • 07:23Results: Representative Chemokine-induced T Cell Polarization
  • 09:30Conclusion

Summary

Automatic Translation

Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.

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