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从复杂的动物相关样品测序宏基因组和Metatranscriptomes:净化不纯
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Please note that all translations are automatically generated.
Click here for the English version.
从复杂的动物相关样品测序宏基因组和Metatranscriptomes:净化不纯
DOI:
10.3791/52117-v
•
11:23 min
•
December 22, 2014
•
Yan Wei Lim
,
Matthew Haynes
,
Mike Furlan
,
Charles E. Robertson
,
J. Kirk Harris
,
Forest Rohwer
1
Department of Biology
,
San Diego State University
,
2
DOE Joint Genome Institute
,
3
Department of Molecular, Cellular and Developmental Biology
,
University of Colorado
,
4
Department of Pediatrics, School of Medicine
,
University of Colorado
Chapters
00:05
Title
02:01
Sample Collection and Pre-treatment
04:11
Generating Viral Metagenome and Viral-like Particle Enrichment and Purification
07:12
Generating Microbial Metagenome
08:57
Results: Generation of Viral and Microbial Metagenomes
10:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
使用囊性纤维化气道为例,手稿呈现全面的工作流包括宏基因组和metatranscriptomic方法的组合来表征动物相关样品中的微生物和病毒的社区。
Tags
Metagenomics
Metatranscriptomics
Host-associated Microbiome
Cystic Fibrosis
Sputum
DNA Extraction
RNA Extraction
High-throughput Sequencing
Microbial Community
Viral Community
Taxonomy
Functional Analysis
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