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可视化的非溶胞胞吐作用<em>新型隐球菌</em>从巨噬细胞用数码光学显微镜
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
DOI:

10:02 min

October 21, 2014

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Chapters

  • 00:05Title
  • 01:27Isolation and Preparation of Primary Bone Macrophages from C57/B16 Mice
  • 04:51Co‐incubation of Primary Murine Macrophages and C. neoformans
  • 06:56Visualizing Non‐lytic Exocytosis in Real‐time Utilizing Digital Light Microscopy
  • 08:11Results: Primary Murine Macrophages Undergo Different Types of Non‐lytic Exocytosis of C. neoformans
  • 09:36Conclusion

Summary

Automatic Translation

我们将介绍如何可视化巨噬细胞C。新型隐球菌 (Cn)的相互作用进行实时,特别强调采用数字光学显微镜非溶胞胞吐过程。单独使用这种技术被感染的巨噬细胞可以研究,以确定这种现象的各个方面。

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