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Immunology and Infection
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通过胞内细菌,研究内溶酶体系统的改造荧光纳米粒子中的应用
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Immunology and Infection
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Immunology and Infection
Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
通过胞内细菌,研究内溶酶体系统的改造荧光纳米粒子中的应用
DOI:
10.3791/52058-v
•
11:38 min
•
January 02, 2015
•
Yuying Zhang
,
Viktoria Krieger
,
Michael Hensel
1
Abteilung Mikrobiologie, Fachbereich Biologie/Chemie
,
Universität Osnabrück
Chapters
00:05
Title
01:26
Synthesis of 10-nm Gold Nanoparticles (Gold NPs)
02:28
Coating of Gold NPs with BSA and Labeling with Rhodamine N-hydroxysuccinimidyl Ester (NHS)
04:30
Culture of HeLa Cells and Salmonella Bacteria
05:40
Infection of HeLa Cells by Salmonella and Gold-BSA-Rhodamine NPs Pulse Chase-labeling
07:04
Imaging and Image Analysis
09:18
Results: Salmonella Infection Rearranges the Endolysosomal System in HeLa Cells
10:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了合成和纳米粒子的荧光标记(NPS)的方法。所述纳米颗粒是在脉冲追踪实验施加标记的真核细胞的内溶酶体系统。内溶酶体系统的细胞内病原体
沙门氏菌
的活动操纵其次是活细胞成像和量化。
Tags
Fluorescent Nanoparticles
Endo-lysosomal System
Intracellular Bacteria
Salmonella Enterica
HeLa Cells
Imaris Software
Confocal Microscopy
Quantitative Analysis
Organelle Labeling
Host-pathogen Interactions
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