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Journal
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Immunology and Infection
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人THP-1巨噬细胞通过核转高效转染
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
Please note that all translations are automatically generated.
Click here for the English version.
人THP-1巨噬细胞通过核转高效转染
DOI:
10.3791/51960-v
•
07:54 min
•
September 02, 2014
•
Marten B. Maeß
,
Berith Wittig
,
Stefan Lorkowski
1
Institute of Nutrition
,
Friedrich Schiller University Jena
Chapters
00:05
Title
01:20
Predifferentiation of THP-1 Macrophage and Preparation for Nucleofection
03:22
Nucleofection of THP-1 Macrophage
04:53
Post-Nucleofection Care
06:15
Gross Characterization of siRNA Transfection THP-1 Macrophages
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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这个协议提出通过电穿孔转染人THP-1巨噬细胞中的siRNA或质粒DNA具有高转染效率,同时保持高的细胞活力和分化和极化全细胞巨噬细胞的能力的有效和可靠的方法。
Tags
Transfection
Human THP-1 Macrophages
Nucleofection
SiRNA
Plasmid DNA
Innate Immune Response
Cell Vitality
Cell Behavior
Cell Differentiation
Cell Polarization
Gene Regulation
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