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焦时间推移成像为牙科复合材料的细胞相容性评价的有效方法
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Please note that all translations are automatically generated.
Click here for the English version.
焦时间推移成像为牙科复合材料的细胞相容性评价的有效方法
DOI:
10.3791/51949-v
•
07:04 min
•
November 09, 2014
•
Ghania Nina Attik
,
Kerstin Gritsch
2
,
Pierre Colon
3
,
Brigitte Grosgogeat
2
1
Laboratoire des Multimatériaux et Interfaces
,
UMR CNRS 5615, Université Lyon1
,
2
UFR d’Odontologie, Université Lyon1; Service de Consultations et de Traitements Dentaires
,
Hospices Civils de Lyon
,
3
UFR d’Odontologie, Université Paris Diderot; Service d’Odontologie
,
APHP, Hôpital Rothschild
Chapters
00:05
Title
01:25
Composite Extract Preparation and Primary Human Gingival Fibroblast (HGF) Cell Culture
02:53
Confocal Time Lapse Images
04:38
Results: Representative Confocal Time Lapse Images of Composite Extract–composed Cell Cultures
06:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
牙科用复合材料的生物相容性研究最多的方面是其细胞毒性三维CLSM时间推移成像结合的荧光信号的量化允许细胞命运的敏感性评价随时间。利用这种方法可能是一种有效的工具,以评估牙科复合材料的相容性。
Tags
Confocal Time-lapse Imaging
Cytocompatibility
Dental Composites
ELS Extra Low Shrinkage
Primary Human Gingival Fibroblasts
Live/Dead Staining
FV10i Confocal System
FV10-ASW 3.1 Software
In Vitro Biocompatibility Assessment
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