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在体外
合成在人体细胞修饰蛋白表达的mRNA的感应
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In Vitro
Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
Please note that all translations are automatically generated.
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在体外
合成在人体细胞修饰蛋白表达的mRNA的感应
DOI:
10.3791/51943-v
•
10:07 min
•
November 13, 2014
•
Meltem Avci-Adali
,
Andreas Behring
,
Heidrun Steinle
,
Timea Keller
,
Stefanie Krajeweski
,
Christian Schlensak
,
Hans P. Wendel
1
Department of Thoracic, Cardiac, and Vascular Surgery
,
University Hospital Tuebingen
Chapters
00:05
Title
01:35
Amplification of Plasmid Inserts and Addition of Poly T-tail by PCR
02:48
In Vitro
Transcription
03:59
Treatment of Purified mRNA with Antartic Phosphatase
05:17
mRNA Transfection of Cells
07:01
Flow Cytometric Analyses of eGFP Expression in Cells
08:14
Results: Modified mRNA is Expressed in Human Cells
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频文章中,我们描述了诱导细胞蛋白表达的
体外
合成修饰的mRNA。
Tags
MRNA Synthesis
In Vitro Transcription
Modified MRNA
Protein Expression
Transfection
HEK293 Cells
EGFP
Regenerative Medicine
Cell Biology
Reprogramming
Article
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