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分离，鉴定和小鼠胸腺上皮细胞的分离纯化
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
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分离，鉴定和小鼠胸腺上皮细胞的分离纯化

DOI:
10.3791/51780-v

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07:20 min

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August 08, 2014

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1Department of Laboratory Medicine & Pathology, Center for Immunology,University of Minnesota

Chapters

  • 00:05Title
  • 01:24Stromal Cell Isolation from Mouse Thymus
  • 03:20TEC Identification, Panning, and Purification
  • 04:48Results: Representative Thymic Epithelial Populations
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了用于分离，鉴定和小鼠胸腺上皮细胞（TECs的）的纯化的有效方法。该协议可以用于胸腺功能的正常T细胞发育，胸腺机能障碍，和T细胞重建研究。

Tags

Thymic Epithelial CellsThymusT Cell DevelopmentCell IsolationCell PurificationCell CharacterizationFlow CytometryCell DepletionCell Sorting

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