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酵母核内体系统的超微结构分析的纳米金标记
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Biology
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Biology
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
Please note that all translations are automatically generated.
Click here for the English version.
酵母核内体系统的超微结构分析的纳米金标记
DOI:
10.3791/51752-v
•
09:49 min
•
July 14, 2014
•
Muriel Mari
,
Janice Griffith
,
Fulvio Reggiori
1
Department of Cell Biology
,
University Medical Center Utrecht
Chapters
00:05
Title
01:21
Spheroplast Preparation
03:07
Nanogold Uptake
03:44
Fixation and Sectioning
04:42
Silver Enhancement for Nanogold Particle Visualization
08:17
Results: Yeast Endosomal Compartments are Visualized by Silver-enhanced Nanogold Labeling
09:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
酵母,
酿酒酵母
,一直是一个关键模式生物识别和研究基因调节内体系统的生物合成和功能。在这里,我们提出了内体车厢的超微结构研究特定的标签一个详细的协议。
Tags
Endosomal System
Yeast
Saccharomyces Cerevisiae
Nanogold Labeling
Ultrastructural Analysis
Spheroplasts
Silver Enhancement
Vesicular Transport
Cell Homeostasis
Pathologies
Cancer
Neurobiological Disorders
Protein Localization
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