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Journal
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Neuroscience
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小鼠皮肤及其毛囊图案和感觉轴突形态分析中的应用平山成像
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
Please note that all translations are automatically generated.
Click here for the English version.
小鼠皮肤及其毛囊图案和感觉轴突形态分析中的应用平山成像
DOI:
10.3791/51749-v
•
13:58 min
•
June 25, 2014
•
Hao Chang
,
Yanshu Wang
,
Hao Wu
,
Jeremy Nathans
2,3
1
Department of Molecular Biology and Genetics
,
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine
,
2
Department of Neuroscience
,
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine
,
3
Department of Ophthalmology
,
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine
Chapters
00:06
Title
02:01
Dorsal Skin for Immunostaining and Melanin Imaging
05:40
Foot Skin for the Analysis of Hair Follicle Patterning
06:42
Foot Skin for Visualizing Sebaceous Glands
07:23
Tail Skin Preparation for the Analysis of Melanin Distribution, Hair Follicle Orientation, and Sebaceous Gland Visualization
08:54
Imaging Hair Follicle Orientation
09:52
Imaging and Tracing Axon Arbors
10:55
Results: Geometry of Various Skin Structures Are Easily Visualized in Skin Flatmounts
12:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
哺乳动物的皮肤中含有结构的多样化 - 如毛囊和神经末梢 - 表现出独特的空间组织模式。作为平坦安装分析皮肤借此组织的2维几何的优点,以产生皮肤结构的全厚的高清晰度图像。
Tags
Mouse Skin
Flat Mount
Hair Follicles
Sensory Axons
Skin Structures
2D Visualization
Optical Sectioning
Skin Anatomy
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