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注重形成:一个基于Cell的检测,以确定一个基因的致癌性
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Medicine
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JoVE Journal
Medicine
Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
Please note that all translations are automatically generated.
Click here for the English version.
注重形成:一个基于Cell的检测,以确定一个基因的致癌性
DOI:
10.3791/51742-v
•
08:18 min
•
December 31, 2014
•
Angel Alvarez
,
Gustavo A. Barisone
,
Elva Diaz
1
Department of Pharmacology
,
University of California, Davis
Chapters
00:05
Title
01:17
Retrovirus Production
03:45
NIH 3T3 Cells and Infection
06:02
Staining and Foci Quantification
07:05
Results: Transcription Factor MXD3 as a Gene of Interest (Potential Oncogene)
07:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
聚焦形成分析提供了一个简单的方法来评估候选基因的转化潜力。
Tags
Focus Formation Assay
Oncogenic Potential
Ras Transformation
NIH 3T3 Cells
Retroviral Transduction
Cell Transformation
Contact Inhibition
Anchorage-independent Growth
Tumor Formation
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