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在
造血干细胞和祖细胞的成年小鼠颅骨骨髓
体内
四维跟踪
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
In Vivo
4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Please note that all translations are automatically generated.
Click here for the English version.
在
造血干细胞和祖细胞的成年小鼠颅骨骨髓
体内
四维跟踪
DOI:
10.3791/51683-v
•
12:54 min
•
September 04, 2014
•
Mark K. Scott
,
Olufolake Akinduro
,
Cristina Lo Celso
1
Department of Life Science and Facility for Imaging by Light Microscopy
,
Imperial College London
,
2
Department of Life Sciences
,
Imperial College London
Chapters
00:05
Title
01:52
Labeling and Injection of HSPCs
03:21
Preparing the Mouse for Imaging
05:20
In Vivo Imaging: High-resolution Stacks and Time-lapse Acquisition
11:25
Results: In Vivo 4D Imaging of HSPCs in Mouse Calvarium
12:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
造血干细胞和祖细胞(HSPCs)和骨髓龛之间的相互作用的性质知之甚少。定制的硬件修改和多步获取协议允许使用双光子共焦显微术图像
体外
骨髓区域内驻留标记HSPCs,跟踪相互作用和运动。
Tags
Hematopoietic Stem Cells
HSCs
Hematopoietic Stem And Progenitor Cells
HSPCs
Bone Marrow Microenvironment
In Vivo Imaging
4-dimensional Tracking
Confocal Imaging
Two-photon Imaging
Osteoblastic Cells
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