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研究DNA环接了单分子荧光共振能量转移
JoVE Journal
Biology
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JoVE Journal
Biology
Studying DNA Looping by Single-Molecule FRET
Please note that all translations are automatically generated.
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研究DNA环接了单分子荧光共振能量转移
DOI:
10.3791/51667-v
•
11:27 min
•
June 28, 2014
•
Tung T. Le
,
Harold D. Kim
1
School of Physics
,
Georgia Institute of Technology
Chapters
00:05
Title
01:21
Double Stranded (ds)DNA Sample Preparation
02:52
Flow Cell Preparation
05:04
Single-molecule Imaging
06:18
Image Processing and Data Analysis
07:48
Determining the J Factor
09:44
Results: Representative Analysis of DNA Looping
10:52
Conclusion
Summary
Automatic Translation
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Automatic Translation
这项研究提出了一个详细的实验步骤,使用单分子荧光共振能量转移(FRET)来衡量的双链DNA循环动力学。该协议还描述了如何提取称为歼因子循环概率密度。
Tags
DNA Looping
FRET
DNA Bending
DNA-protein Interactions
DNA Packaging
Nucleosome
Cyclization
DNA Ligase
DNA Structure
Looping Kinetics
PCR
FRET Pair
Biotin Linker
J Factor
Intrinsic Curvature
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