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重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化<em>大肠杆菌</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from
Escherichia coli
Please note that all translations are automatically generated.
Click here for the English version.
重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化<em>大肠杆菌</em
DOI:
10.3791/51583-v
•
07:35 min
•
June 09, 2014
•
Sarah R. MacEwan*
1,2
,
Wafa Hassouneh*
1,2
,
Ashutosh Chilkoti
2
1
Department of Biomedical Engineering
,
Duke University
,
2
Research Triangle MRSEC
,
Duke University
Chapters
00:05
Title
01:37
Expression of Elastin-like Polypeptides (ELPs)
02:22
Purification of ELPs by Inverse Transition Cycling
04:17
Removal of Contaminants from ELP through Subsequent Rounds of ITC
05:22
Characterization of ELP T
t
by Temperature-programmed Turbidimetry
06:16
Results: Characterization of ELPs Purified from
E. coli
through ITC
07:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
弹性蛋白样多肽是刺激 - 反应的生物聚合物与应用,从重组蛋白纯化给药。这个协议描述了来自
大肠杆菌
的使用其低临界溶解温度的相变行为作为一个简单的替代色谱弹性蛋白样多肽和其肽或蛋白质融合的纯化和表征。
Tags
Elastin-like Polypeptides
Recombinant
Non-chromatographic Purification
Lower Critical Solution Temperature
Phase Transition
Coacervation
Escherichia Coli
Peptide Fusion
Protein Fusion
Biosensing
Drug Delivery
Thermal Properties
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