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体外
聚集测定使用tau蛋白的蛋白质
JoVE Journal
Biology
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JoVE Journal
Biology
In Vitro
Aggregation Assays Using Hyperphosphorylated Tau Protein
Please note that all translations are automatically generated.
Click here for the English version.
体外
聚集测定使用tau蛋白的蛋白质
DOI:
10.3791/51537-v
•
09:22 min
•
January 02, 2015
•
Dexin Sui*
1
,
Mengyu Liu*
1
,
Min-Hao Kuo
1
Department of Biochemistry and Molecular Biology
,
Michigan State University
Chapters
00:05
Title
01:19
Preparations
02:14
Terminal Assay with No Dye
04:44
Continual Mode Assay Using a Plate Reader With Dye
06:14
Continual Mode Assay Using a Compact Spectrofluorometer With Dye
07:33
Results: Tau Aggregation Assayed by Different Modes and Instruments
08:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
未修饰的和磷酸化的tau蛋白以两种
体外
聚集测定中使用以显示磷酸化依赖快速聚合动力学。这些测定铺路用于可调节tau蛋白的倾向,以形成背后阿尔茨海默氏病的进展纤丝化合物未来画面。
Tags
Alzheimer’s Disease
Tauopathies
Hyperphosphorylated Tau
Neurofibrillary Tangles
Tau Aggregation Assays
Recombinant Tau
High-throughput Screens
Tau Aggregates
Neurodegenerative Disorders
Therapeutic Development
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