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采用雾化脂多糖一种产生肺中性粒细胞增多
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
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采用雾化脂多糖一种产生肺中性粒细胞增多

DOI:
10.3791/51470-v

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08:33 min

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December 15, 2014

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, , , ,
1Department of Medicine, Solna and CMM, Respiratory Medicine Unit,Karolinska Institutet, 2Safety Science, Global Regulator Affairs & Patient Safety,AstraZeneca Global Medicines Development

Chapters

  • 00:05Title
  • 01:13Generating an LPS Aerosol
  • 03:14Bronchoalveolar Lavage (BAG)
  • 05:12Formalin Fixation of Lung Tissue for Histological Assessment
  • 06:26Results: Representative Pulmonary Neutrophilia Data
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述诱导中性粒细胞肺部炎症通过挑战脂多糖雾化通过雾化，建模急性肺损伤的方法。此外，基本的手术技术对于肺隔离，气管插管和支气管肺泡灌洗也有所说明。

Tags

Pulmonary NeutrophiliaAerosolized LipopolysaccharideAcute Lung InjuryExperimental ModelPseudomonas AeruginosaBronchoalveolar LavageSterile Pulmonary Inflammation

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