A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Cell and Developmental Biology and Program in Developmental Biology,Vanderbilt University Medical Center, 2Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology,Cincinnati Children's Hospital Medical Center, 3Vanderbilt Ingram Cancer Center,Vanderbilt University School of Medicine
Chapters
- 00:05Title
- 01:41Depletion from Xenopus Extract
- 02:57Optimizing Xenopus Extract for β-catenin Degradation
- 04:10Radiolabeled β-catenin Degradation Assay in Xenopus Egg Extract
- 05:47β-catenin-luciferase Degradation Assay in Xenopus Egg Extract
- 07:24Results: Analysis of β-catenin Degradation
- 09:03Conclusion
Se describe un método para el análisis de la degradación de proteínas usando radiomarcado y proteínas de fusión luciferasa-en extracto de huevo de Xenopus y su adaptación para la selección de alto rendimiento para moduladores de moléculas pequeñas de la degradación de proteínas.
