JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
人中性粒细胞流动室粘附实验
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Human Neutrophil Flow Chamber Adhesion Assay
Please note that all translations are automatically generated.
Click here for the English version.
人中性粒细胞流动室粘附实验
DOI:
10.3791/51410-v
•
12:42 min
•
July 02, 2014
•
Yebin Zhou
,
Dennis F. Kucik
3,4
,
Alexander J. Szalai
,
Jeffrey C. Edberg
1
Genetics and Genomic Sciences Graduate Program
,
University of Alabama at Birmingham
,
2
Birmingham Veterans Affairs Medical Center
,
3
Department of Pathology
,
University of Alabama at Birmingham
,
4
Department of Biomedical Engineering
,
University of Alabama at Birmingham
,
5
Department of Medicine
,
University of Alabama at Birmingham
Chapters
00:05
Title
01:50
HUVEC Layer Preparation
03:07
Purified Ligand Coating
05:11
Neutrophil Separation
08:33
Flow Chamber Adhesion Assay
10:20
Results: The Flow Chamber Assay Enables Analyses of Adhesive Properties of Human Peripheral Blood Neutrophils to HUVECs and Purified Ligand Substrates
11:49
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
报道定量中性粒细胞粘附的方法。此方法创建类似于血管遇到了动态流环境。它使中性粒细胞粘附到任何纯化黏附分子(配体)或类似
体内
环境与剪切应力的上下文内皮细胞基板(HUVEC)的调查。
Tags
Neutrophil Adhesion
Endothelial Cells
Inflammation
β2 Integrin
ICAM
Autoimmune Disease
Flow Chamber
Shear Stress
HUVEC
Adhesion Receptors
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
到哺乳动物的上皮
细胞在
体外细菌粘附的含量
人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具
分离人脐静脉内皮细胞和水流条件下其使用中的中性粒细胞轮回研究
量<em在体外</em>激活的主人微血管内皮细胞在静态条件下测量的检测,以中性粒细胞粘附
一个流程粘附实验来研究白细胞招聘剪切应力的人力肝窦内皮细胞条件下
简化人力中性粒细胞胞外陷阱(英语教师)分离和处理
在剪切粘结力的测定T淋巴细胞粘附分子相互作用的研究
小鼠与人肺泡巨噬细胞的分离和
体外
培养
动态粘附法在炎症性肠病抗粘连治疗功能分析中的作用
全骨髓的制备,用于中性粒系细胞的大规模细胞测定分析
Read Article