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Immunology and Infection
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HIV逆转录酶和整合酶抑制剂的快速筛选
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
Please note that all translations are automatically generated.
Click here for the English version.
HIV逆转录酶和整合酶抑制剂的快速筛选
DOI:
10.3791/51400-v
•
05:46 min
•
April 09, 2014
•
Steven J. Smith
,
Stephen H. Hughes
1
HIV Drug Resistance Program
,
National Cancer Institute
Chapters
00:05
Title
01:09
Viral Stock Generation
02:29
Compound Screening
03:42
Measurement of Luciferase Activity
04:41
Results: Representative Luciferase Signal Readout Inhibition
05:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里我们描述了细胞的细胞毒性和单轮感染性测定允许对化合物的快速,准确的筛选以确定它们的细胞毒性(CC
50)
和IC
50
值对WT和耐药性的HIV-1。
Tags
HIV Reverse Transcriptase
HIV Integrase
Antiviral Compounds
Drug Resistance
Cytotoxicity Assay
Luciferase Reporter
Viral Infectivity
Screening Platform
High-throughput Screening
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