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Journal
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Biology
/
一个
在体内
交联方法分离蛋白复合物从
果蝇
胚胎
JoVE Journal
Biology
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JoVE Journal
Biology
An
in vivo
Crosslinking Approach to Isolate Protein Complexes From
Drosophila
Embryos
Please note that all translations are automatically generated.
Click here for the English version.
一个
在体内
交联方法分离蛋白复合物从
果蝇
胚胎
DOI:
10.3791/51387-v
•
08:51 min
•
April 23, 2014
•
Ming Gao
,
Patrick McCluskey
,
Sudan N. Loganathan
,
Alexey L. Arkov
1
Department of Biological Sciences
,
Murray State University
Chapters
00:05
Title
01:14
Preparing Large Apple Juice Agar Plates
02:04
Collecting Embryos and Pre-crosslinking Treatment
04:35
In vivo Crosslinking
05:34
Preparing Proteins and Immunoprecipitation
07:10
Results: Crosslinked Tud Protein Complex Analysis
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
多组分蛋白复合物的细胞功能和发展过程中发挥关键作用。在这里,我们描述了用于从
果蝇
胚胎分离的天然蛋白质复合物后,随后交联的复合物用于随后的结构-功能分析的纯化
在体内
交联的方法。
Tags
In Vivo Crosslinking
Protein Complexes
Drosophila Embryos
Immunopurification
Tudor Protein Complex
Germline Development
Mass Spectrometry
Protein Purification
Multisubunit Protein Complexes
Native Environment
Cell Lysis
Formaldehyde
Methylated Arginines
Methylated Lysines
Article
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