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单病毒原子力显微镜和超分辨率荧光成像的样品制备

JoVE Journal
Biology

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JoVE Journal Biology

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

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单病毒原子力显微镜和超分辨率荧光成像的样品制备

DOI:

10.3791/51366-v

•

05:31 min

•

January 02, 2014

•

Jeffery A Hodges², Saveez Saffarian²

¹Department of Physics & Astronomy,University of Utah, ²Center for Cell and Genome,University of Utah

Chapters

00:05Title
00:52Coverslip Cleaning in Preparation for Chemical Treatment
01:36Formation of the PLL-g-PEG Thin Film Layer
02:36Avidin Binding Enhancement
03:20Active Face Antibody Tether
04:24Results: Single Virion Imaging
05:06Conclusion

Summary

Automatic Translation

通过超分辨率荧光成像或原子力显微镜（AFM）将病毒附着在表面上是单一病毒成像的要求。在这里，我们演示了一种样品制备方法，用于控制将病毒粘附到适合用于 AFM 和超分辨率荧光成像的玻璃表面。

Tags

Sample Preparation Single Virion Atomic Force Microscopy Super-resolution Fluorescence Imaging Immobilization Glass Surface PLL-g-PEG PLL-g-PEG-Biotin Avidin Biotinylated Antibodies Virus-specific

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