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Immunology and Infection
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在伴有海藻酸钠生产新基因的鉴定<em>铜绿假单胞菌</em>使用小型<em> himar1</em>水手转座子介导的诱变
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Identification of Novel Genes Associated with Alginate Production in
Pseudomonas aeruginosa
Using Mini-
himar1
Mariner Transposon-mediated Mutagenesis
Please note that all translations are automatically generated.
Click here for the English version.
在伴有海藻酸钠生产新基因的鉴定<em>铜绿假单胞菌</em>使用小型<em> himar1</em>水手转座子介导的诱变
DOI:
10.3791/51346-v
•
08:57 min
•
March 10, 2014
•
T. Ryan Withers
,
Yeshi Yin
,
Hongwei D. Yu
1
Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine
,
Marshall University
Chapters
00:05
Title
01:42
Preparation of Bacterial Strains and Biparental Conjugation
04:42
Detection and Isolation of Mucoid Colonies
06:00
gDNA Restriction Digestion and Ligation and Inverse PCR and Sequence Analysis
07:07
Results: Mini-
himar1
Mariner Transposon-mediated Mutagenesis
08:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里我们描述了使用微型
himar1
水手转座子介导的诱变以产生高密度的插入突变体文库,以屏幕的协议,分离和鉴定中的原型的
铜绿假单胞菌
菌株PAO1新颖藻稳压器。
Tags
Pseudomonas Aeruginosa
Alginate
Mucoidy
Mini-himar1 Transposon
Mutagenesis
MucE
KinB
AlgU
MucA
Cystic Fibrosis
Biofilms
Virulence Genes
Colony Morphology
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