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癌症干细胞从人类前列腺癌样本隔离

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Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

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癌症干细胞从人类前列腺癌样本隔离

DOI:

10.3791/51332-v

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07:16 min

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March 14, 2014

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Samuel J. Vidal, S. Aidan Quinn, Janis de la Iglesia-Vicente, Dennis M. Bonal, Veronica Rodriguez-Bravo, Adolfo Firpo-Betancourt, Carlos Cordon-Cardo, Josep Domingo-Domenech

¹Department of Pathology,Icahn School of Medicine at Mount Sinai, ²Molecular Biology Program,Memorial Sloan-Kettering Cancer Center

Chapters

00:05Title
01:17Harvesting and Processing of Human Prostrate Cancer Tissue
02:18Generation of Cell Suspensions from Tissue Sections
04:07Isolation of Prostrate Cancer Stem Cells (CSCs) by FACS
05:56Results: Representative Human Prostrate Cancer Cell Populations
06:50Conclusion

Summary

Automatic Translation

肿瘤干细胞（肿瘤干细胞）直接从人体组织中的隔离是必要的其生物学特性。这个手稿描述了从人体组织前列腺肿瘤干细胞的分离的方法，同时还提供疑难排解困难的步骤提示。

Tags

Cancer Stem Cells Prostate Cancer HLA Class I Flow Cytometry Human Tumor Samples Cell Isolation Molecular Characterization Functional Validation Immunodeficient Mice

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